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Product

Electrolytic Respiration-Based Biodegradation Tester

Electrolytic Respiration-Based Biodegradation Tester

Brand: Thmorgan
Model: T9000C

I. Product Applications

1. Can be used for biobased biodegradability testing, meeting the requirements of GB/T.   19276-1;

2. Suitable for biodegradability testing using the seawater method, GB/T   40611-2021;

3. Can be used for potential biodegradation tests of materials under the action of specific microorganisms, GB/T 19275-2003;

4. Can be used for rapid bio-degradability testing of chemicals, in accordance with GB/T 21801, GB/T 21802, and GB/T 21803;

5. Meets international standards such as OECD301C, OECD301F, OECD302C, EC-partc4, and ISO5815-1:2003;

6. Meets international standards for testing and research, including OECD, EEC, ASTM, BBA, ISO, and MITI.

7. Can be used for the determination of BOD3, BOD5, BOD7, BOD28, BOD42, and more;

8. Can be used for biodegradation testing in the activated sludge process;

     9. Can be used for microbial respiration measurement;

II. Product Features

1. Determine the actual oxygen consumption of the sample by measuring charge movement;

2. No sample dilution required: The maximum detection limit can reach 10,000 mg;

3. Continuous electrolytic oxygen supply: Each bottle of electrolyte can provide several years of electrolytic oxygen; simply add fresh electrolyte once the current electrolyte is depleted to continue use.

4. Precise Temperature Control: Water bath temperature control with a set temperature range of 5–99℃ and a temperature adjustment accuracy of ±0.1℃.

5. Number of channels: Each instrument includes 9 detection channels;

6. Magnetic stirring system: Provides long-term stirring;

7. Data output system: processed by dedicated software on a computer, with an LCD display;

8. Automatic liquid level control: Automatically replenishes water to prevent dry running;

III. Product Principle

Microorganisms consume the oxygen in the culture flask, producing carbon dioxide. The calcium oxide absorbs this carbon dioxide, causing the pressure inside the culture flask to decrease. This pressure difference between the two sides of the pressure gauge triggers the electrolytic circuit to close. As a result, the copper sulfate solution in the electrolytic cell undergoes electrolysis, generating oxygen that is replenished into the culture flask, gradually restoring the flask’s pressure to its initial level. The amount of electric charge passed through the electrolytic cell is directly proportional to the amount of oxygen produced. A coulometer precisely measures the electric charge flowing into the electrolytic cell, thereby allowing us to calculate the oxygen consumption. The ratio of the actual oxygen consumption to the theoretical oxygen consumption represents the biodegradation rate of the sample under test.

 

 

IV. Technical Parameters

1. Working conditions:

1.1 Power supply voltage: AC 220V ±10%, 50Hz, single-phase;

1.2 Operating temperature: 15–30℃;

1.3 Relative humidity: ≤80%;

2. Oxygen supply system:

2.1 Oxygen Supply Method: Closed-loop copper sulfate electrolysis oxygen supply;

2.2 Internal liquid in the electrolytic cell: saturated copper sulfate solution;

2.3 Opening and closing of electrolysis: Air pressure on both sides of the U-tube;

2.4 Electrolytic Accuracy: Sensitivity 9.806 Pa; internally filled with a saturated copper sulfate solution.

2.5 Electrodes: Platinum (positive electrode), Copper (negative electrode);

2.6 Electrolytic cell material: High-borosilicate hard glass;

3. Temperature control system:

3.1 Temperature control method: Water bath;

3.2 Set temperature range: 5–99℃, with a step size of 0.1℃;

3.3 Temperature accuracy: ≤ ±0.1℃;

4. Reaction system:

4.1 Reaction method: Liquid-phase reaction;

4.2 Reaction System: Closed-electrolysis reaction;

4.3 Stirring Method: 9-channel magnetic stirring;

4.4 Culture flask volume: 500 ml;

4.5 Decarbonization System: None;

4.6 Condensation System: None;

5. Detection system:

5.1 Detection Principle: The coulometric method detects charge movement, where four charges correspond to one oxygen molecule.

5.2 Current measurement range: 0–140 mA;

5.3 Detection accuracy ≤ ±0.001 mA;

6. Software control system:

6.1 Software control system with independent intellectual property rights;

6.2 The software collects data and can generate real-time data curves.

6.3 Hosting System: Professional editions of operating systems such as Windows 7/8/10;

6.4 Data Storage Method: Data can be stored in real time during the experiment;

6.5 Equipped with a cloud platform for remote data access;

 

V. Standard Configuration

 

VI. Typical Cases

This is a bio-degradation instrument of the T9000C type, using the electrolytic respiration method, and complies with GB/T.   The experimental results for determining the biodegradability of a certain sample, according to the method standard 19276.1-2003, were obtained over an experimental period of 59 days. At the end of the experiment, the degradation rate of cellulose was 89.53%. The curves for each sample group were smooth, and the growth had essentially come to an end. The experimental design and the graph of the experimental results are shown below:

Table 1: Experimental Design

Figure 1: Oxygen Consumption Curve

 

Electrolytic Respiration-Based Biodegradation Tester

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  • Beijing Thmorgan Biotechnology Co., Ltd

    Telephone：4000-688-151

  • Mailbox：info@thmorgan.com

    Address：2711, building 2, Beijing big data intelligent industrial park, 6 Liye Road, Huilongguan town, Changping District, Beijing

  • Tel: 4000-688-151