Product


Real-time fluorescence quantitative PCR instrument

Real-time fluorescence quantitative PCR instrument

Brand: Thmorgan
Model: Q6

I. Product Applications

1. Quantitative/qualitative gene expression analysis;

2. Melting curve analysis;

3. SNP genotyping;

4. MicroRNA analysis;

5. Gene mutation analysis;

6. Quality control graphic analysis;

7. Calculation of PCR amplification efficiency;

8. Detection of various diseases including HBV, HCV, HIV, HPV, SARS, H1N1, AIDS, and CSF;

9. Various types of research, including genomics, infectious disease studies, stem cell research, research on tumors and genetic disorders, and drug research;

 

II. Product Features

1. 6-color fluorescence detection channel, enabling six-color fluorescence detection;

2. 96-well plate design, allowing up to 96 samples to be processed in a single run;

3. Utilizing six-point temperature control technology and edge thermal compensation technology, this system effectively enhances temperature control accuracy and temperature uniformity, and allows for gradient setting.

4. Advanced semiconductor temperature-control technology with fast heating and cooling rates, while ensuring temperature accuracy within ±0.1℃ and temperature uniformity within ±0.2℃.

 

 

III. Technical Parameters

1. Basic parameters:

1.1 Sample capacity: 96 × 0.2 ml;

1.2 Applicable consumables: 0.2 ml single tubes, 8×0.2 ml strip tubes, 96-well plates;

1.3 Reaction system: 5–120 μl;

1.4 Operating environment temperature: 15–30℃;

1.5 Storage temperature: -20–55℃;

1.6 Relative ambient humidity: ≤85%;

1.7 Size and Weight: 35.0 (W) × 42.0 (D) × 46.0 (H) ,25 kg;

1.8 Power supply: 100–240V, 50–60Hz, 1500VA;

 

2. Temperature control module:

2.1 Heating Method: Peltier heating method, with 6 independent temperature control channels and intelligent temperature control technology;

2.2 Temperature control range: 4℃–100℃ (the hot lid features automatic pressure adjustment);

2.3 Heating rate: 4.0℃/s;

2.4 Cooling rate: 4.0℃/s;

2.5 Temperature control accuracy: ±0.1℃;

2.6 Temperature uniformity: ±0.2℃;

2.7 Number of temperature control zones: 6 independently controlled zones, combined with thermal compensation technology to minimize temperature edge effects.

2.8 Gradient Temperature Function: Available;

2.9 Number of temperature gradient columns: 12;

2.10 Temperature gradient range: 1℃–32℃;

2.11 Gradient temperature zones: 6;

2.12 Gradient temperature selection range: 30℃–100℃ (when room temperature is below 28℃)

 

3. Optical module:

3.1 Excitation light source: LED;

3.2 Detection component: PD;

3.3 Excitation/Detection Channel Propagation Medium: Motion Scanning;

3.4 Excitation/Detection Channel Count: 5 (expandable to 6 channels);

3.5 Excitation Wavelength:

First channel: 470 nm ± 10 nm;

Second channel: 525 nm ± 10 nm;

Third channel: 570 nm ± 10 nm;

Fourth channel: 620 nm ± 10 nm;

Fifth channel: 670 nm ± 10 nm;

The sixth channel supports user customization;

3.6 Detection Wavelength:

First channel: 512 nm - 528 nm;

Second channel: 562 nm - 578 nm;

Third channel: 612nm–628nm;

Fourth channel: 662nm–678nm;

Fifth channel: 702 nm – 718 nm;

Sixth channel: Supports user customization;

3.7 Applicable dyes and probes: FAM/SYBR Green I, VIC/HEX/CY3, ROX/Texas Red, Cy5, TAMRA;

3.8 Detection sensitivity: ≥1 copy;

3.9 Confidence level: 99.90%;

3.10 Resolution: Single-response as low as 1.5-fold change;

3.11 Sample Repeatability: CV ≤ 1%;

3.12 Linear Range: 1–10 10

3.13 Sample linearity: ≥0.99;

 

4. Software module:

4.1 Software Language: Switchable between Chinese and English;

4.2 Control Mode: Operates via touchscreen control; data analysis performed by an external computer; supports multi-device connectivity and can be linked to LIMS or LIS systems.

4.3 Software Functions: Real-time monitoring, automatic identification and calculation of positive and negative results, automatic generation of standard curves, absolute/relative quantification, multiplex quantification, melting curve analysis, gene mutation detection, targeted genotyping (Taqman probe method), Tm value determination, quality control graph analysis, PCR amplification efficiency, and more.

4.4 Data Output Formats: EXCEL/WORD/PDF;

4.5 Qualifications: CE/ISO9001/ISO13485;

 

IV. Configuration

1. One host machine;

2. One set of software;

3. One set of materials;

Real-time fluorescence quantitative PCR instrument