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Product

Real-time fluorescent quantitative PCR instrument

Real-time fluorescent quantitative PCR instrument

Brand: Thmorgan
Model: Q3

I. Product Applications

1. Quantitative/qualitative gene expression analysis;

2. Melting curve analysis;

3. SNP genotyping;

4. MicroRNA analysis;

5. Gene mutation analysis;

6. Quality control graphic analysis;

7. Calculation of PCR amplification efficiency;

8. Various types of research, including genomics, infectious disease studies, stem cell research, research on tumors and genetic disorders, and drug research;

9. Detection of various diseases including HBV, HCV, HIV, HPV, SARS, H1N1, AIDS, and CSF;

II. Product Features

1. Three-color fluorescence detection channel, enabling triple fluorescence detection;

2. 96-well plate design, allowing up to 96 samples to be processed in a single run;

3. Employing three-point temperature control technology and edge thermal compensation technology, this system effectively enhances temperature control accuracy and temperature uniformity, and allows for gradient settings.

4. Advanced semiconductor temperature-control technology featuring high heating and cooling rates, while ensuring temperature accuracy within ±0.1℃ and temperature uniformity within ±0.2℃.

III. Technical Parameters

1. Basic parameters:

1.1 Sample capacity: 96 × 0.2 ml;

1.2 Applicable consumables: 0.2 ml single tubes, 8×0.2 ml strip tubes, 96-well plates;

1.3 Reaction system: 10–120 μl;

1.4 Operating environment temperature: 15–30℃;

1.5 Storage temperature: -20–55℃;

1.6 Relative ambient humidity: ≤85%;

1.7 Size and Weight: 35.0 cm (W) × 42.0 cm (D) × 46.0 cm (H) ,19 kg;

1.8 Power supply: 220V 50-60Hz;

2. Temperature control module:

2.1 Heating Method: Peltier heating method, with three independent temperature control channels and intelligent temperature control technology;

2.2 Temperature control range: 4℃–100℃ (the hot lid features automatic pressure adjustment);

2.3 Heating rate: 4.0℃/s;

2.4 Cooling rate: 4.0℃/s;

2.5 Temperature control accuracy: ±0.1℃;

2.6 Temperature uniformity: ±0.2℃;

3. Optical module:

3.1 Excitation light source: LED;

3.2 Detection component: PD;

3.3 Excitation/Detection Channel Propagation Medium: Motion Scanning;

3.4 Number of excitation/detection channels: 3;

3.5 Excitation Wavelength:

First channel: 470 nm ± 10 nm;

Second channel: 570 nm ± 10 nm;

Third channel: 620 nm ± 10 nm;

3.6 Detection Wavelength:

First channel: 520 nm ± 10 nm;

Second channel: 628 nm ± 10 nm;

Third channel: 670 nm ± 10 nm;

3.7 Applicable dyes and probes: FAM/SYBR Green I, ROX/Texas Red, Cy5;

3.8 Detection sensitivity: ≥1 copy;

3.9 Confidence level: 99.90%;

3.10 Resolution: Single-response variation as low as 1.5-fold;

3.11 Sample Repeatability: CV ≤ 1%;

3.12 Linear Range: 1–10 10；

3.13 Sample linearity: ≥0.99;

4. Software module:

4.1 Software Language: Switchable between Chinese and English;

4.2 Control Mode: Operates via touchscreen control; data analysis performed by an external computer; supports multi-device networking and can be connected to LIMS or LIS systems.

4.3 Software Functions: Real-time monitoring, automatic identification and calculation of positive and negative results, automatic generation of standard curves, absolute/relative quantification, multiplex quantification, melting curve analysis, gene mutation detection, targeted genotyping (Taqman probe method), Tm value determination, quality control graph analysis, PCR amplification efficiency, and more.

4.4 Data output formats: EXCEL/WORD/PDF.

IV. Configuration

1. One host machine;

2. One set of software;

3.  A set of materials.

Real-time fluorescent quantitative PCR instrument

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Focus on us

Focus on us

  • Beijing Thmorgan Biotechnology Co., Ltd

    Telephone：4000-688-151

  • Mailbox：info@thmorgan.com

    Address：2711, building 2, Beijing big data intelligent industrial park, 6 Liye Road, Huilongguan town, Changping District, Beijing

  • Tel: 4000-688-151