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Product

Nucleic Acid Extraction and Fluorescent Quantitative Integrated Instrument

Nucleic Acid Extraction and Fluorescent Quantitative Integrated Instrument

Brand: Thmorgan
Model: GPQ2000

I. Product Applications

1. Integrated automation for sample loading, nucleic acid extraction, and fluorescent quantitative PCR analysis of various viruses, with fully automated operation throughout the entire workflow—sample in, results out.

2. It can automatically perform sample loading, nucleic acid extraction, and qPCR detection for human blood, body fluids, hair, muscle, and other samples.

3. It can automatically perform sample loading, nucleic acid extraction, and qPCR detection for samples such as animal and plant tissues, microorganisms, and cells.

4. Suitable for gene expression studies: quantitative and qualitative gene expression analysis, SNP genotyping, microRNA analysis, methylation analysis, and more;

5. Suitable for melting curve analysis;

6. Suitable for high-throughput sample screening;

II. Product Features

1. Integrated design: a single instrument can automatically perform sample opening and collection, nucleic acid extraction, qPCR detection, and qPCR report generation.

2. Highly automated end-to-end workflow: From sample loading and nucleic acid extraction to qPCR detection and report generation, the entire process is fully automated with no manual intervention required.

3. The entire system operates in a closed-loop configuration, ensuring zero contact between laboratory personnel and samples for safe handling;

4. High throughput: capable of processing 1–96 samples at a time;

5. The magnetic-bead-based nucleic acid extraction method offers high efficiency and stable purity.

6. The qPCR detection module features six-color fluorescence detection channels and exhibits high detection sensitivity.

7. High efficiency: Sample testing can be completed in just 2 hours;

8. High adaptability: accommodates various types of samples and tests;

9. Implement zoning design, UV sterilization, HEPA filtration, and other measures to minimize contamination to the greatest extent possible;

III. Operating Principle:

The instrument comprises six modules: an autosampler module, a fully automated nucleic acid extraction module, an automated liquid-handling module, an automated sealing module, a qPCR detection module, and a report-generation module. The instrument can automatically open lids and collect samples; it employs a magnetic-bead-based method for fully automated nucleic acid extraction. Following extraction, the nucleic acid sample is automatically transferred to reaction tubes via the liquid-dispensing and sampling module and mixed with pre-loaded assay reagents to establish the PCR reaction mix. The automated sealing module then seals the PCR plates, after which the qPCR detection module automatically performs amplification and fluorescence signal detection. Finally, the instrument automatically generates and outputs the qPCR test report. With seamless integration among all modules, the entire nucleic acid testing process is fully automated, truly achieving “sample in, report out.”

IV. Technical Parameters

(1) Whole Machine System

1. Whole-system configuration: The system comprises six modules—automatic sample-loading module, nucleic acid extraction module, automated liquid-handling module, sealing module, qPCR detection module, and report-output module.

2. Control System: Built-in touch display;

3. Data Interfaces: 2 USB ports;

4. External dimensions: 150 cm (W) × 65 cm (D) × 95 cm (H);

5. Voltage: 220 V, 50 Hz;

6. Operating temperature: 15–30°C;

7. Open consumables design: compatible with standard consumables;

(2) Auto-Sampling Module

1. Automatic Cap-Opening/Closing Function: Supports automatic capping and uncapping of sample tubes, eliminating the need for manual operation;

2. Automatic pipetting function: high-precision automated pipetting, supporting sample transfer;

3. Automatic lid opening and automatic pipetting are integrated for seamless, end-to-end operation;

4. Sample quantity: 1–96;

5. Sample volume: 20–1000 µL;

(3) Nucleic Acid Extraction Module

1. Extraction principle: magnetic bead method;

2. Sample size: 1–96;

3. Single sample volume: 20–1000 μl;

4. Magnetic bead recovery rate: ≥99%;

5. Pore-to-pore variation after purification: CV ≤ 1%;

6. Anti-pollution design: zoned design + UV sterilization + HEPA filtration ；

7. Module configuration: adopts a rotary design;

(4) Automated Pipetting Module

  1. Pipetting range: 2–100 μL;

2. Pipetting performance: accuracy ≤ 1%, repeatability CV ≤ 1%;

3. Pipetting channels: 8-channel pipetting unit;

4. Work envelope: The robotic arm supports flexible multi-axis motion in the vertical, fore-and-aft, and lateral directions, with precise and reliable positioning, ensuring full coverage of the operational workspace.

(5) PCR Plate Sealing Module

1. Sealing principle: roller-pressing type;

2. Film-sealing method: pressure-sensitive adhesive;

3. Film material: high-transmittance optical fluorescence detection-grade film;

4. Film-sealing time: 8 s;

5. The film-sealing run track shares the same running track as the qPCR detection run track;

(6) qPCR Detection Module

1. PCR reaction volume: 20–100 µL;

2. Sample capacity: 96 × 0.2 ml;

3. Sample repeatability: CV ≤ 1%;

4. Detection channels: 6 channels;

5. Compatible dyes: FAM, HEX, ROX, Cy5, TAMRA, and Quasar 705;

6. Excitation Light Source: Long-life, high-power LED lamp (full spectrum);

7. Temperature range: 4°C–100°C (with automatic pressure regulation via the heated lid);

8. Heating rate: 4.0°C/s;

9. Cooling rate: 4.0°C/s;

10. Temperature accuracy: ±0.1℃;

11. Temperature uniformity: ±0.2°C.

 

V. Configuration

Serial Number

Name

Quantity

Unit

1

Auto-Sampling Module

1

set

2

Nucleic Acid Extraction Module

1

set

3

Automatic Pipetting Module

1

set

4

PCR plate Sealing Module

1

set

5

qPCR detection module

1

set

6

Report Output Module

1

set

7

Software

1

set

  

Nucleic Acid Extraction and Fluorescent Quantitative Integrated Instrument

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Focus on us

Focus on us

  • Beijing Thmorgan Biotechnology Co., Ltd

    Telephone：4000-688-151

  • Mailbox：info@thmorgan.com

    Address：2711, building 2, Beijing big data intelligent industrial park, 6 Liye Road, Huilongguan town, Changping District, Beijing

  • Tel: 4000-688-151