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Product

Benchtop associative cell bioreactor

Benchtop associative cell bioreactor

Brand ：Thmorgan
Model ：CF6000

Thmorgan Benchtop Cell Bioreactor is a cell culture system suitable for all process development. The system adopts the latest mechanical processing and manufacturing technology to meet the needs of users in suspension cell culture and adherent cell culture, inherits the flexibility and parallelization of small biorereactors, and simple module selection or configuration changes can meet the diversified needs of scientific research users in application scenarios, and also meet the compliance requirements of pharmaceutical users.

 

Use of a product

1. Suitable for conventional mammalian cell culture, insect cell culture, plant cell culture and other cell types, can meet the suspension, microcarrier and perfusion culture process requirements.

2. Suitable for antibody process development;

3. In line with (c)GMP environment, it can cover the needs of cell therapy, vaccines, stem cells, exosomes and other applications.

4. Suitable for fast process transformation in food, medicine and chemical industry;

 

Second, product characteristics

1. Convenient comparison: multi-row design, observation experiment is more intuitive;

2. High safety: selection of high-quality components to ensure the quality of the reactor;

3. High degree of intelligence: intuitive measurement and control system, can automatically and accurately adjust CO2 concentration, pH, DO, temperature and other parameters;

4. Using double-layer Marine slurry with low shear force, it has good fluidity and low shear force to protect cells from damage during culture;

5. Multi-functional top cover interface: pH, DO, temperature, inoculation port, defoaming, acid, alkali, feed and other interfaces;

6. Off-position sterilization: autoclave sterilization can be used, acid and alkali resistant;

7. Data can be graphically displayed: PLC as a control system, reliable, stable, the same screen display operating parameters.

 

Principle of three products

This product belongs to artificial intelligence bioreactor. Artificial intelligence control technology, online gas detection technology, online liquid detection technology, the whole process to simulate the microbial growth environment, according to the online gas and liquid detection indicators, at any time to adjust the reaction system of carbon dioxide concentration, temperature, dissolved oxygen, stirring speed and other microbial growth parameters, the whole process without manual participation. It has good fluidity and low shear force to protect cells from damage during culture. The efficient, accurate and intelligent reactor continues to work to ensure the continuous reproduction and growth of microorganisms in the reactor, so as to meet the cell and microbial culture needs of different users.

 

Four technical parameters

1. Tank system:

1.1 Tank capacity: 3L, 5L, 15L three specifications, filling coefficient of 30 ~ 70%;

1.2 Tank material: high temperature and high pressure borosilicate glass and 316L high-quality stainless steel, surface polishing finish Ra ≤ 0.4μm;

1.3 Tank size:

 

1.4 Design pressure: 0.3Mpa

1.5 Top cover: PH, DO, inoculation, pipetting, temperature, displacement, feeding and other interfaces;

1.6 Sterilization method: off-position sterilization in autoclave;

 

2. Mechanical mixing system:

2.1 Mechanical mixing: The two-layer low shear Marine impeller tri-inclined blade is designed, and the diameter of the mixing blade is 52mm, 64mm, 88mm; Top stirring;

2.2 Mechanical drive: microcomputer intelligent PID control servo motor;

2.3 Control range: PID digital display 5~500rpm, stirring speed is continuous, stable and adjustable, accuracy: ±1rpm;

 

3. Gas control system:

3.1 Measurement method: thermal gas mass flowmeter;

3.2 Control mode: four-gas control system (N2, O2, CO2, Air), air compressor to provide air, solenoid valve with mass flow meter control air flow rate, ventilation 0.1 ~ 10L/min;

3.3 Filtration method: 37mm 0.2μm PTFE inlet filter; 51mm 0.2μm PTFE tail gas filter;

 

4. Gas distributor: enhance gas-liquid mass transfer efficiency

 

    3L 5L 15L
Gas distributor Big Bubble 7holes-①0.8mm 8holes-①0.8mm 9holes-①0.8mm
Xiao Pao 40-50μm 40-50μm 40-50μm

 

 

5. Temperature control system:

5.1 Measurement method: based on PT100 temperature sensor;

5.2 Temperature control: microcomputer intelligent PID control tank external heating blanket heating;

5.3 Cooling control: built-in condensing pipe, microcomputer intelligent PID control, water inlet connected to the water pipe head, condensate water out of the water outlet;

5.4 Control range: PID digital display; 3L specifications are +5℃ ~ 65℃, 5L and 15L are +5℃ ~ 70℃; Accuracy: ±0.1℃;

 

6.pH control system:

6.1 Determination method: MeTTler/Hamilton pH electrode sensor; Cascade with acid, base, CO2;

6.2 Control mode: microcomputer intelligent PID control and acid-base bottle connected peristaltic pump;

6.3 Control range: PID digital display 0 ~ 12, accuracy: ±0.01;

 

Peristaltic pump Peristaltic pump type 114DV*2+400A*2(Programmable)
speed 114DV:0-200rpm    400A:0-200rpm
Current Speed 114DV:0.02ml/min-52.6ml/min
400A:0.0025ml/min-17.5ml/min
Peristaltic pump tube diameter 114DV:φ2.4mm*1.6mm(Inner diameter * wall thickness)(19#Silica gel tube)
400A:φ2mm*1mm(Inner diameter * wall thickness)(Silica gel tube)

 

7.DO control system:

7.1 Determination method: based on polarographic dissolved oxygen electrode;

7.2 Control mode: cascade control of dissolved oxygen electrode, O2, N2, Air, flow rate and speed;

7.3 Control range: PID digital display 0 ~ 200%, accuracy: ±1%;

 

8. Foam level control system:

8.1 Measurement method: Foam sensing based on highly adjustable conductivity;

8.2 Control mode: mechanical + automatic defoaming;

8.2.1 Mechanical defoaming: The rake defoaming device is installed slightly above the fermentation liquid surface and rotated with the stirring shaft;

8.2.2 Automatic defoaming: microcomputer intelligent PID control peristaltic pump connected with defoaming agent;

 

9. Software control system:

9.1 Software control system with independent intellectual property rights, each tank can be independently controlled;

9.2 Online detection and control of temperature, pH and other related parameters in the fermentation process;

9.3 Software data collection, can generate real-time data curve;

9.4 Data storage mode: It can be stored in real time in the experiment;

9.5 Can be connected to the upper computer and printer to achieve remote monitoring and data storage, export and printing.

 

Five standard configurations

Benchtop associative cell bioreactor

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  • Beijing Thmorgan Biotechnology Co., Ltd

    Telephone：4000-688-151

  • Mailbox：info@thmorgan.com

    Address：2711, building 2, Beijing big data intelligent industrial park, 6 Liye Road, Huilongguan town, Changping District, Beijing

  • Tel: 4000-688-151